Transient expression Improving agroinfiltration-based transient gene ⦠The resulting expression vectors were then introduced into A. tumefaciens strain GV3101 by electroporation.. 2.3. 2016 Jun 16;7:743. doi: 10.3389/fpls.2016.00743. Plants | Free Full-Text | A Putative Effector LtCSEP1 from ... Nicotiana benthamiana is a suitable transient system for ⦠Nicotiana benthamiana as a transient expression host to produce ⦠The expression of cancer/testis NY-ESO-1 antigen was detected in N. benthamiana leaves using a PVX-based transient assay. Here we use a transient expression system in the model plant Nicotiana benthamiana to reconstitute the two-step plant-derived biosynthetic pathway for auxin (indole acetic acid) to achieve accumulation up to 500 ng/g fresh mass (FM). Transient expression in Nicotiana benthamiana fluorescent marker lines provides enhanced definition of protein localization, movement and interactions in planta. 1 Deletions in the Repertoire of Pseudomonas syringae pv. Transient Expression 2022 May 13;13:912293. doi: 10.3389/fpls.2022.912293. Nicotiana benthamiana plants were cultivated at 25/20°C in a greenhouse with a photoperiod of 18 h light/6 h dark. While much recent attention has focussed on improving vector design to increase agroinfiltration-based transient expression levels, few â¦
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